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AmeriDx® H. pylori Antigen - Recombinant VacA Protein (21232112), 1 mg

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Product Details
Summary
$299.00
21232112
In stock

Minimum quantity for "AmeriDx® H. pylori Antigen - Recombinant VacA Protein (21232112), 1 mg" is 1.

Note
 

Cytotoxin associated gene A (CagA), recombinant CagA protein, cagA2d domain

Product Description
 

Catalog Number: 21232112

Size: 100 ug

Source: E. coli derived H. pylori VacA 

             NCBI ID: AF049653

Sequences:


Host: E. coli

Format: Dry Powder

Reactivity: Immune assay with AmeriDx® H. pylori VacA antibodies.

QC Testing: Concentration Measurement, SDS-PAGE Analysis.

Formulation and Purity

Recombinant VacA protein over-expressed in E coli strain BL21, and affinity purification from cell pellet by Ni-NTA resin, and dialysis against 1x PBS. The protein is > 97% pure, as determined by SDS-PAGE. The concentration is measured by Nanodrop.

Storage Buffer: In PBS before lyophilized

Preparation and Storage

Upon received, save at freezer immediately if not used. After initial reconstitution with DI water (50-100uL), aliquot if necessary, and save the product at -80°C for future use.

Description

VacA is a pore-forming toxin, help H. pylori to form channels in intracellular sites on host cells. The host cells are  gastric epithelial cells, parietal cells, T cells, and other types of immune cells. 

Product Notices

  • Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  • Please refer to www.ameridx.com/ for technical protocols.

References

1.  Foegeding NJ, Caston RR, McClain MS, Ohi MD, Cover TL. An Overview of Helicobacter pylori VacA Toxin Biology. Toxins (Basel). 2016 Jun 3;8(6):173. doi: 10.3390/toxins8060173. PMID: 27271669; PMCID: PMC4926140.

 

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