Catalog Number: 21232112
Size: 1 mg / 10 mg
Source: E. coli derived H. pylori VacA
NCBI ID: AF049653
Host: E. coli
Format: Dry Powder
Reactivity: Immune assay with AmeriDx® H. pylori VacA antibodies.
QC Testing: Concentration Measurement, SDS-PAGE Analysis.
Formulation and Purity
Recombinant VacA protein over-expressed in E coli strain BL21, and affinity purification from cell pellet by Ni-NTA resin, and dialysis against 1x PBS. The protein is > 97% pure, as determined by SDS-PAGE. The concentration is measured by Nanodrop.
Storage Buffer: In PBS before lyophilized
Preparation and Storage
Upon received, save at freezer immediately if not used. After initial reconstitution with DI water (50-100uL), aliquot if necessary, and save the product at -80°C for future use.
VacA is a pore-forming toxin, help H. pylori to form channels in intracellular sites on host cells. The host cells are gastric epithelial cells, parietal cells, T cells, and other types of immune cells.
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.ameridx.com/ for technical protocols.
1. Foegeding NJ, Caston RR, McClain MS, Ohi MD, Cover TL. An Overview of Helicobacter pylori VacA Toxin Biology. Toxins (Basel). 2016 Jun 3;8(6):173. doi: 10.3390/toxins8060173. PMID: 27271669; PMCID: PMC4926140.
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