Catalog Number: 21232111
Size: 1 mg / 10 mg
Source: E. coli derived H. pylori KatA (Catalase) Protein
NCBI ID: WP_000247318
GSNKDVKQTTAFGAPVWDDNNVITAGPRGPVLLQSTWFLE KLAAFDRERIPERVVHAKGSGAYGTFTVTKDITKYTKAKI FSKVGKKTECFFRFSTVAGEKGSADAVRDPRGFAMKYYTE EGNWDLVGNNTPVFFIRDAIKFPDFIHTQKRDPQTNLPNP DMVWDFWSNVPESLYQVTWVMSDRGIPKSFRHMDGFGSHT FSLINAKGERFWVKFHFETMQGVKHLTNEEAAEVRKYDPD SNQRDLFDAIAGGDFPKWKMSIQVMPEEDAKKYRFHPFDV TKIWYLQDYPLMEVGIVELNKNPENYFAEVEQAAFTPANV VPGIGYSPDRMLQGRLFSYGDTHRYRLGVNYPQIPVNRPR CPFHSSSRDGYMQNGYYGSLQNYTPSSLPGYKEDKSARDP KFNLAHIEKEFEVWNWDYRAEDSDYYTQPGDYYRSLPADE KERLYDTIGGSLAHVTHKEIVDKQLEHFKKADPKYAEGVK KALEKHQKMMKDMHAKDMHHMKKKK
Host: E. coli
Format: Dry Powder
Reactivity: Immune assay with AmeriDx® H. pylori antibodies.
QC Testing: Concentration Measurement, SDS-PAGE Analysis.
Formulation and Purity
Recombinant protein was over-expressed in E coli strain BL21, and affinity purified from cell pellet by Ni-NTA resin, and dialysis against 1x PBS. The protein is > 97% pure, as determined by SDS-PAGE. The concentration is measured by Nanodrop.
Storage Buffer: In PBS before lyophilized
Preparation and Storage
Upon received, save at freezer immediately if not used. After initial reconstitution with DI water (50-100uL), aliquot if necessary, and save the product at -80°C for future use.
Serum Catalase (KatA) and AhpC antibodies are associated with GC risk and KatA may serve as a biomarker for GC. KatA/FlaA combined analysis improved screening accuracy.
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.ameridx.com/ for technical protocols.
1. Zhang B, Li HL, Fan Q, Guo F, Ren XY, Zhou HB, Zhu JW, Zhao YS, Tian WJ. Serum Helicobacter pylori KatA and AhpC antibodies as novel biomarkers for gastric cancer. World J Gastroenterol. 2016 Jun 7;22(21):5060-7. doi: 10.3748/wjg.v22.i21.5060. PMID: 27275098; PMCID: PMC4886381.
Product Insert can be after login.