CATALOG NUMBER: 21233337
SIZE: 100 ug/1 mg/2 mg
Synonyms: Neurofilament light polypeptide, 68 kDa neurofilament protein, neurofilament triplet L protein
PROTEIN INFO: Full length NF-L protein purified from insect cells
Accession ID: P07196
DESCRIPTION
Neurofilament consists of three major components: neurofilament light polypeptide (NF-L), mediate polypeptide (NF-M), and heavy polypeptide (NL-H). NF-L has 542 amino acid including a 91 aa N-terminal head, a 304 aa alpha -helical rod, and a 147 aa C-terminal tail[1]. NF-L is first reported as a 69 kDa protein from by Hoffman and Lasek in 1975 analyzing the axonal transport paradigm[2], it plays an important role in the maintenance of neuronal caliber and possibly the pathogenesis of motor neuron diseases[3, 4].
SEQUENCES
MSSFSYEPYYSTSYKRRYVETPRVHISSVRSGYSTARSAYSSYSAPVSSSLSVRRSYSSS SGSLMPSLENLDLSQVAAISNDLKSIRTQEKAQLQDLNDRFASFIERVHELEQQNKVLEA ELLVLRQKHSEPSRFRALYEQEIRDLRLAAEDATNEKQALQGEREGLEETLRNLQARYEE EVLSREDAEGRLMEARKGADEAALARAELEKRIDSLMDEISFLKKVHEEEIAELQAQIQY AQISVEMDVTKPDLSAALKDIRAQYEKLAAKNMQNAEEWFKSRFTVLTESAAKNTDAVRA AKDEVSESRRLLKAKTLEIEACRGMNEALEKQLQELEDKQNADISAMQDTINKLENELRT TKSEMARYLKEYQDLLNVKMALDIEIAAYRKLLEGEETRLSFTSVGSITSGYSQSSQVFG RSAYGGLQTSSYLMSTRSFPSYYTSHVQEEQIEVEETIEAAKAEEAKDEPPSEGEAEEEE KDKEEAEEEEAAEEEEAAKEESEEAKEEEEGGEGEEGEETKEAEEEEKKVEGAGEEQAAK KKD
FORMAT: Dry Powder
Predicted Molecular Mass: 61 kDa
REACTIVITY: Immune assay with AmeriDx® NF-L antibodies: B0220112, B0220113, B0220114, B0220115, B0220116, B0220117, 0220118.
FORMULATION AND PURITY
Recombinant protein is over-expressed in Drosophila s2 cells, and purified by Ni-NTA resin, and dialysis against 1x PBS. The protein is > 97% pure, as determined by SDS-PAGE. The concentration is measured at O.D280nm by Nanodrop.
STORAGE BUFFER: In PBS before lyophilized
RECONSTITUTION AND STORAGE
Upon received, save at freezer immediately if not used. After initial reconstitution with DI water (50-100uL), aliquot if necessary, and save the product at -80°C for future use.
APPLICATION NOTES
n/a
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.ameridx.com/ for technical protocols.
References
1. Adebola, A.A., et al., Neurofilament light polypeptide gene N98S mutation in mice leads to neurofilament network abnormalities and a Charcot-Marie-Tooth Type 2E phenotype. Hum Mol Genet, 2015. 24(8): p. 2163-74.
2. Hoffman, P.N. and R.J. Lasek, The slow component of axonal transport. Identification of major structural polypeptides of the axon and their generality among mammalian neurons. Journal of Cell Biology, 1975. 66(2): p. 351-366.
3. Xu, Z., et al., Increased expression of neurofilament subunit NF-L produces morphological alterations that resemble the pathology of human motor neuron disease. Cell, 1993. 73(1): p. 23-33.
4. Monteiro, M.J., et al., Expression of NF-L in both neuronal and nonneuronal cells of transgenic mice: increased neurofilament density in axons without affecting caliber. J Cell Biol, 1990. 111(4): p. 1543-57.
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