Please sign in so that we can notify you about a reply
HE4 Antigen (Cat# 21231209)
WAP four-disulfide core domain protein 2, also known as Epididymal secretory protein E4, Major epididymis-specific protein E4, Putative protease inhibitor WAP5, WFDC2, and HE4, is a secreted protein containing two WAP domains. WFDC2/HE4 is a member of a family of stable 4-disulfide core proteins that are secreted at high levels. It is expressed in a number of normal tissues, including those of the male reproductive system and regions of the nasopharynx andrespiratory tract. It is highly expressed in a number of tumor cell lines, such ovarian, colon, breast, lung, and renal. It was initially described as being exclusively transcribed in the epididymis. WFDC2 may be a component of the innate immune response in the lung, nasal, and oral cavities, thereby suggesting that WFDC2 functions in concert with related WAP domain containing proteins in the epithelial host defenses. WFDC2 re-expression in lung carcinomas may prove to be associated with the tumor type and should be studied in further detail. Mammary gland expression of tammar WFDC2 during the course of lactation showed WFDC2 was elevated during pregnancy, reduced in early lactation, and absent in mid-late lactation. WFDC2 / HE4 can undergo a complex series of alternative splicing events that can potentially yield five distinct WAP domain containing protein isoforms.
- Bingle,L. et al., 2002, Oncogene. 21 (17):2768-73.
- HellstrÃ¶m,I. et al., 2003, Cancer Res. 63 (13):3695-700.
- Bingle,L. et al., 2006, Respir Res. 7 : 61.
- Galgano,M.T. et al., 2006, Mod Pathol.19 (6):847-53.
- Sharp,J.A. et al., 2007, Evol Dev. 9 (4): 378-92.
HE4 Protein Product Information
RP3-461P17.2, EDDM4, WFDC2, MGC57529, WAP5, dJ461P17.6
A DNA sequence encoding the human WFDC2 (NP_006094.3) (Met 1-Phe 124) was expressed, with a N-terminal polyhistidine tag
HE4 QC Testing Results
> 95 % as determined by SDS-PAGE
Recombinant Human HE4 Antigen
Samples are stable for up to twelve months from date of receipt at -70â?/p>
The recombinant HE4 protein comprises of124 amino acids with a predicted molecular mass of 13 kDa. The apparent molecular mass of rHE4 is approximately 15 kDa in SDS-PAGE under reducing conditions
In sterile PBS, pH 7.4, with 0.02% sodium azide.
Store it under sterile conditions at -70â? It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
A hard copy of COA with reconstitution instructions is sent along with the products. Please refer to it for more detailed information.