AmeriDx® qEIATM Human AMH ELISA Kit (colorimetric, 96 wells, E31231222 )
The AmeriDx® qEIATM human Anti-Mullerian Hormone (AMH) ELISA Kit is a designed for the quantitative measurement of Human AMH protein in serum, plasma, tissue homogenates.
The Human AMH is synthesized by the granulosa cells of the preantral and early developing antral follicles, belongs to the transforming growth factor beta superfamily [1]. AmH is a hormone involving sex organs development in wombs, and a ovary biomarker for reproduction diseases such as polycystic syndrome[2]. it's primary physiological function in the ovary is to prevent primordial follicles recruitment and regulate FSH secretion in the early follicular phase [3].
Specificity | This assay has high sensitivity and excellent specificity for detection of human AMH antigen (P03971) in human serum, plasma, tissue homogenates. |
| Standard Curve Range | 62.5 pg/mL – 4,000 pg/mL
|
| Sensitivity | 15.6 pg/mL |
| Detection Method | colorimetric |
| Assay Range | inter-assay cv: <12% intra-assay cv: <10% |
| Sample Volume | 50-100 uL |
| Shipped in | wet ice |
| Storage Temperature | 4oC |
This product is for research use only in US.
Kit Components
- Human AMH Capture Antibody coated ELISA plate, 12 x 8-well strips, Item Number: 31231222P (1)
- Human AMH Standards, Item Number: 21231235
- AMH-CAL-A (1)
- AMH-CAL-B (1)
- AMH-CAL-C (1)
- AMH-CAL-D (1)
- AMH-CAL-E (1)
- qEIAmix detection Antibody Mix, Item Number: 21231235qM (1)
- ELISA Substrate Solution, Item Number: ESUBS22 (1)
- Concentrate Wash Buffer, Item Number: CWBuffer22 (1)
- Concentrate Assay Diluent, Item Number: CADiluent22 (1)
- Stop Solution, Item Number: ESTOP22 (1)
- Product Insert, M021620174, (1).
Reference
1. Pellatt L, Hanna L, Brincat M, et al. Granulosa cell production of anti-Müllerian hormone is increased in polycystic ovaries. J Clin Endocrinol Metab. 2007; 92(1): 240–245, doi: 10.1210/jc.2006-1582. PMID: 17062765.
2. Ozay AC, Emekcı Ozay O, Gulekli B. Comparison of Anti-müllerian Hormone (AMH) and Hormonal Assays for Phenotypic Classification of Polycystic Ovary Syndrome. Ginekol Pol. 2020;91(11):661-667. doi: 10.5603/GP.a2020.0122. PMID: 33301159.
3. La Marca A, Broekmans FJ, Volpe A, et al. ESHRE Special Interest Group for Reproductive Endocrinology--AMH Round Table. Anti-Mullerian hormone (AMH): what do we still need to know? Hum Reprod. 2009; 24(9): 2264–2275. PMID: 19520713.