E-MOTM Poliovirus Antibody ELISA Kit (Item No E20142012)
Poliovirus can cause life-threatening diseases once infected in the human body without vaccination. Most of the people in the world are immunized with polio virus vaccine by either OPV or IPV during their early age. However the antibody titer maintain around 20 years after the first immunization, after that the infection risk comes back. A boost shot of polio vaccine is needed to re-activate the protection mechanism. The kit is designed to monitor the antibody titer in human serum or other body fluids. Big data in 2019 SARS CoV-2 virus (COVID-19) pandemic showed that poliovirus vaccination is negatively proportional to COVID-19 infection[1].
GeneScan Diagnostics’ AmeriDx® ELISA Kit is a Sandwich Enzyme-Linked Immunosorbent Assay. A polio virus antigen first coated on a
96-well plate. Standards, positive controls, negative controls and samples are added to the wells, and antibody in the samples binds to the immobilized capture antigen. Next, a HRP conjugated goat anti human IgG/A/M antibody is added, producing an antigen-antibody-HRP antibody “sandwich”. Then ELISA Substrate Solution is added, produces a blue color with its intensity proportional to the concentration of antibody from the sample bound to the antigen on plate. Finally, the Stop solution changes the reaction color from blue to yellow, and the microwell absorbance is read at 450 nm and 650 nm with a microplate reader.
| Specificity | This assay has high sensitivity and excellent specificity for detection of human antibodies recognizing polio virus RdRp protein. |
| Standard Curve Range | 20 pg/mL - 200 pg/mL |
| Sensitivity | n/a |
| Assay Type | Indirect ELISA |
| Inter-Assay | n/a |
| Intra-Assay | n/a |
| Sample Volume | 50 - 100 uL |
| Kit Type | ELISA Kit (Colorimetric) |
shipped in: blue ice
storage temperature: 4°C.
This product is for research use only in US, and for diagnostics device in Europe with CE mark.
Kit components
- Assay plate (8x 12 strips)
- Standard
- HRP-Goat anti-human IgG/IgM/IgA (100 x concentrate)
- Antibody Diluent
- Stop Solution
- Sample Diluent
- Wash Buffer (25 x concentrate)
- TMB Substrate
- Instruction manual
1. Comunale, B. A., et al. (2021). "Poliovirus Vaccination Induces a Humoral Immune Response That Cross Reacts With SARS-CoV-2." Frontiers in Medicine 8(1285).