AmeriDx® Human CA125 ELISA Kit (Item No E21231217 )
Cancer antigen 125 (CA125) is a protein found on the surface of many ovarian cancer cells. It also can be found in other cancer types as well as in small amounts in normal tissue.
CA125 is used as a tumor marker, which means the test can help determine the presence of various cancer types. Most often, the CA125 test is used to monitor the effectiveness of an ovarian cancer treatment and to check if the ovarian cancer has returned.
America Diagnostics’ AmeriDx® Human CA125 Antigen ELISA Kit is a Sandwich Enzyme-Linked Immunosorbent Assay. A human CA125 specific mouse monoclonal antibody is first coated on a 96-well plate. Standards and samples are then added to the wells. The CA125 binds to the immobilized capture antibody. Next, a biotinylated mouse monoclonal anti-human CA125 detection antibody is added, producing an antibody-antigen-antibody “sandwich”. Avidin-horseradish peroxidase (Avidin-HRP) is subsequently added, followed by ELISA Substrate Solution, producing a blue color. The blue color intensity is proportional to the concentration of CA125 present in the sample. Finally, the addition of the Stop solution changes the reaction color from blue to yellow, and the microwell absorbance is read at 450 nm and 650 nm with a microplate reader.
|Specificity||This assay has high sensitivity and excellent specificity for detection of human CA125 antigen in human serum samples.|
|Standard Curve Range||15 U/mL - 500 U/mL|
|Assay Type||Indirect ELISA|
|Sample Volume||50 - 100 uL|
|Kit Type||ELISA Kit (Colorimetric)|
shipped in: blue ice
storage temperature: 4°C.
This product is for research use only in US.
- Human CA125 Capture Antibody coated ELISA plate, 12 x 8-well strips (1)
- Human CA125 Standards:
- CAL A CA125 0 U/mL, 8 mL (1)
- CAL B CA125 19 U/mL, 0.75 mL (1)
- CAL C CA125 56 U/mL, 0.75 mL (1)
- CAL D CA125 167 U/mL, 0.75 mL (1)
- CAL E CA125 500 U/mL, 0.75 mL (1)
- 20X Avidin-HRP, 0.75 mL (1)
- 10X Wash Buffer Concentrate, 25 mL (1)
- ELISA Substrate Solution, 12 mL (1)
- Stop Solution, 12 mL (1)
- Instruction manual (1)