AmeriDx® Human MSLN ELISA Kit (Item No E30142009 )
Human Mesothelin is normally expressed on mesothelial cells lining the pleura, pericardium, peritoneum, and in the serum. However, the function of Mesothelin is not known. It is a potentially useful biomarker for certain cancers, particularly mesotheliomas, ovarian adeno- carcinomas, pancreatic adenocarcinomas, squamous cell carcinomas, and lung cancers. As such, it has become the focus of cancer research.
America Diagnostics’ AmeriDx® Human Mesothelin Antigen ELISA Kit is a Sandwich Enzyme-Linked Immunosorbent Assay. A human Mesothelin specific mouse monoclonal antibody is first coated on a 96-well plate. Standards and samples are then added to the wells. The Mesothelin binds to the immobilized capture antibody. Next, a biotinylated mouse monoclonal anti-human Mesothelin detection antibody is added, producing an antibody-antigen-antibody “sandwich”.
Avidin-horseradish peroxidase (Avidin-HRP) is subsequently added, followed by the addition of ELISA Substrate Solution, producing a blue color. The blue color intensity is proportional to the concentration of Mesothelin present in the sample. Finally, the addition of Stop solution changes the reaction color from blue to yellow, and the microwell absorbance is read at 450 nm and 650 nm with a microplate reader.
| Specificity | This assay has high sensitivity and excellent specificity for detection of human MSLN antigen in human serum samples. |
| Standard Curve Range | 2 pg/mL - 100 pg/mL |
| Sensitivity | 1 pg/mL |
| Assay Type | Indirect ELISA |
| Inter-Assay | n/a |
| Intra-Assay | n/a |
| Sample Volume | 50 - 100 uL |
| Kit Type | ELISA Kit (Colorimetric) |
shipped in: blue ice
storage temperature: 4°C.
This product is for research use only in US.
Kit components
- Human MSLN Capture Antibody coated ELISA plate, 12 x 8-well strips (1)
- Biotinylated conjugated Human Mesothelin Detection Antibody, 15 mL (1)
- Human MSLN Standards:
- CAL A MSLN 0 pM, 8 mL (1)
- CAL B MSLN 3.7 pM, 0.75 mL (1)
- CAL C MSLN 11.1 pM, 0.75 mL (1)
- CAL D MSLN 33.3 pM, 0.75 mL (1)
- CAL E MSLN 100 pM, 0.75 mL (1)
- 20X Avidin-HRP, 0.75 mL (1)
- 10X Wash Buffer Concentrate, 25 mL (1)
- ELISA Substrate Solution, 12 mL (1)
- Stop Solution, 12 mL (1)
- Instruction manual (1)