AmeriDx® FOB ELISA Kit (colorimetric, 96 wells, E30142001 )

AmeriDx® FOB ELISA Kit (colorimetric, 96 wells, E30142001 )

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AmeriDx® FOBELISA Kit (Item No E30142001 )

AmeriDx® Human Fecal Occult Blood (FOB) Antigen ELISA Kit is designed for the quantification of human hemoglobin in stool samples. This kit specifically measures human hemoglobin without cross-reaction to animal blood. The test is useful for detecting gastrointestinal bleeding and in the aid of screening for colorectal adenoma/polys and cancer, as well as other inflammatory bowel diseases.

Fecal hemoglobin detection is recommended by America Cancer Society (ACS), Center for Disease Control and Prevention (CDC) and Center for Medical Service (CMS) of the Department of Health of the United States. The Fecal Occult Blood (FOB) Rapid Test has been used in the past 40 years. Immunochemical FOB test dramatically increases the analytical sensitivity and specificity in the detection of human hemoglobin in feces compared with Guaiac tests, which is supported by a few clinical trials. However, these rapid tests do not give an insight to the severity of the bleeding in the lower gastrointestinal system.

This AmeriDx® FOB assay using human hemoglobin-specific antibodies would bring significant advantages over the qualitative rapid FOB tests. The advantages are two folders: 1) does not require dietary restrictions such as no raw meat, vitamin C rich food (salad, fruits, etc.), 2) 100-fold lower limit of detection (LoD) than the guaiac FOB test, 3) potentially limits the    false-negative results due to highly specific human hemoglobin antibodies are used.



This assay has high sensitivity and excellent specificity for detection of FOB in human stool samples.

Standard Curve Range

30 ng/mL – 800 ng/mL


30 ng/mL

Detection Method


Assay Range

inter-assay cv: < 4%

intra-assay cv: <10%

Sample Volume

50-100 uL

Shipped in

wet ice

Storage Temperature


This product is for research use only in US.

Kit Components

  1. Human FOB Capture Antibody coated ELISA plate, 12 x 8-well strips, Item Number: 30142001P (1)
  2. Human FOB Standards, Item Number: FOB-CAL
    • FOB-CAL-A (1)
    • FOB-CAL-B (1)
    • FOB-CAL-C (1)
    • FOB-CAL-D (1)
    • FOB-CAL-C (1)
  3. FOB detection Antibody Mix, Item Number: 30142001DB (1)
  4. ELISA Substrate Solution, Item Number: ESUBS22 (1)
  5. Concentrate Wash Buffer, Item Number: CWBuffer22 (1)
  6. Concentrate Assay Diluent, Item Number: CADiluent22 (1)
  7. Stop Solution, Item Number: ESTOP22 (1)
  8. Instruction manual, M021620173, (1).