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Human Vitamin B12 (VB12) ELISA Kit
This assay is a direct competitive inhibition enzyme immunoassay. Antibody specific for Vitamin B12 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells with biotin-conjugated VB12. A competitive inhibition reaction is launched between VB12 (Standards or samples) and biotin-conjugated VB12 with the pre-coated VB12 antibody. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in opposite to the amount of VB12 bound in the initial step. The color development is stopped and the intensity of the color is measured.
| Specificity | This assay has high sensitivity and excellent specificity for detection of human VB12. No significant cross-reactivity or interference between human VB12 and analogues was observed. |
| Standard Curve Range | 1.56 pg/mL - 100 pg/mL |
| Sensitivity | 1.498 pg/mL |
| Assay Type | Competitive ELISA |
| Inter-Assay | %CV < 10 |
| Intra-Assay | %CV < 8 |
| Sample Volume | 50 - 100 uL |
| Kit Type | ELISA Kit (Colorimetric) |
shipped in: blue ice
storage temperature: 4°C.
This product is for research use only in US, and for diagnostics device in Europe with CE mark.
Kit components
- Assay plate (8x 12 strips)
- Standard
- HRP-avidin (100 x concentrate)
- HRP-avidin Diluent
- Stop Solution
- Biotin-conjugate Diluent
- Biotin-conjugate (10 x concentrate)
- Sample Diluent
- Wash Buffer (25 x concentrate)
- TMB Substrate
- Instruction manual