AmeriDx® Human HE4 ELISA Kit (Item No E21231218 )
Human epididymis protein 4 (HE4), a relatively new marker for ovarian carcinoma, is the product of the WFDC2 (HE4) gene that is overexpressed in patients with ovarian carcinoma. HE4 levels correlates with clinical status (progression vs no progression) in 70% of the samples.
This ELISA Kit is used to detect HE4levels in serum or other bodily fluid in healthy patients or patients with epithelial ovarian cancer.
America Diagnostics’ AmeriDx® Human HE4 Antigen ELISA Kit is a Sandwich Enzyme-Linked Immunosorbent Assay. A human HE4 specific mouse monoclonal antibody is first coated on a 96-well plate. Standards and samples are then added to the wells. The HE4 binds to the immobilized capture antibody. Next, a biotinylated mouse monoclonal anti-human HE4 detection antibody is added, producing an antibody-antigen-antibody “sandwich”. Avidin-horseradish peroxidase (Avidin-HRP) is subsequently added, followed by ELISA Substrate Solution, producing a blue color. The blue color intensity is proportional to the concentration ofHE4 present in the sample. Finally, the addition of the Stop solution changes the reaction color from blue to yellow, and the micro well absorbance is read at 450 nm and 650 nm with a microplate reader.
|Specificity||This assay has high sensitivity and excellent specificity for detection of human HE4 antigen in human serum samples.|
|Standard Curve Range||2 pg/mL - 100 pg/mL|
|Assay Type||Indirect ELISA|
|Sample Volume||50 - 100 uL|
|Kit Type||ELISA Kit (Colorimetric)|
shipped in: blue ice
storage temperature: 4°C.
This product is for research use only in US.
- Human HE4 Capture Antibody coated ELISA plate, 12 x 8-well strips (1)
- Human HE4 Standards:
- CAL A HE4 0 pM, 8 mL (1)
- CAL B HE4 2.73 pM, 0.75 mL (1)
- CAL C HE4 5.25 pM, 0.75 mL (1)
- CAL D HE4 10.5 pM, 0.75 mL (1)
- CAL E HE4 21 pM, 0.75 mL (1)
- 20X Avidin-HRP, 0.75 mL (1)
- 10X Wash Buffer Concentrate, 25 mL (1)
- ELISA Substrate Solution, 12 mL (1)
- Stop Solution, 12 mL (1)
- Instruction manual (1)