AmeriDx® Human IgG total ELISA Kit, Cat No EH2013095
The Human IgG total ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for detection of IgG total in human plasma, serum, and cell culture samples. This assay employs a quantitative sandwich enzyme immunoassay technique that measures human IgG total in approximately 3 hours. A polyclonal antibody specific for human IgG total has been pre-coated onto a 96-well microplate with removable strips. IgG total in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for human IgG total, which is recognized by a streptavidin-peroxidase (SP) conjugate. All unbound material is washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.
| Specificity | Reacts with: Human IgG total |
| Standard Curve Range | 780 - 50000 ng/mL |
| Sensitivity | 390 ng/mL |
| Assay Type | Indirect ELISA |
| Sample Type | Cell culture supernatant, Serum, Plasma |
| Intra-Assay | n/a |
| Sample Volume | 50 - 100 uL |
| Kit Type | ELISA Kit (Colorimetric) |
shipped in: blue ice
storage temperature: 4°C.
This product is for research use only in US, and for diagnostics device in Europe with CE mark.
Kit components
- Assay plate (8x 12 strips)
- Standard
- HRP-Goat anti-human IgG/IgM/IgA (100 x concentrate)
- Antibody Diluent
- Stop Solution
- Sample Diluent
- Wash Buffer (25 x concentrate)
- TMB Substrate
- Instruction manual