AmeriDx® Human GDF11 ELISA KIT, Cat No E30142010
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Growth Differentiation Factor 11 (GDF11). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Growth Differentiation Factor 11 (GDF11). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Growth Differentiation Factor 11 (GDF11), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Growth Differentiation Factor 11 (GDF11) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
| Specificity | Reacts with: Human GDF11/BMP-11 |
| Standard Curve Range | 0.15 - 100 ng/mL |
| Sensitivity | 0.15 ng/mL |
| Assay Type | Indirect ELISA |
| Sample Type | Cell culture supernatant, Serum, Plasma |
| Intra-Assay | n/a |
| Sample Volume | 50 - 100 uL |
| Kit Type | ELISA Kit (Colorimetric) |
shipped in: blue ice
storage temperature: 4°C.
This product is for research use only in US.
Kit components
- Assay plate (8x 12 strips)
- Standard
- HRP-Goat anti-human IgG/IgM/IgA (100 x concentrate)
- Antibody Diluent
- Stop Solution
- Sample Diluent
- Wash Buffer (25 x concentrate)
- TMB Substrate
- Instruction manual