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AmeriDx® 2X SYBR Green Real-Time PCR Master Mix, 1.0 mL/500RXNs

500 rxns for $264

List price: $290.00
$264.99
You save: $25.01 (9%)
N/A
AG3326
In stock
+
Our quantity discounts:
Quantity 2+ 5+
Price $484.00 $1,056.00

Minimum quantity for "AmeriDx® 2X SYBR Green Real-Time PCR Master Mix, 1.0 mL/500RXNs" is 1.

This 2 x concentration premix is used for SYBR Green I fluorescence fitting method for real-time quantitative PCR. The optimization product contains HotStart concentration GeneScan Taq DNA Polymerase, SYBR Green I, dNTPs, Mg2+, reaction buffer, and stabilizer component. This product is mainly used to detect cDNA target sequence after reverse transcription of genomic DNA target sequence and RNA. Before heating, anti-Taq monoclonal antibody binds to Taq enzyme and inhibits Taq enzyme activity thereby suppressing the occurrence of non-specific hybridization of template DNA and primers and the primer-dimer induced non-specific amplification at low temperatures. Anti-Taq monoclonal antibodies are completely inactivated in the denaturation step of a PCR's first cycle and Taq Polymerase activity is not hindered therefore greatly improving the sensitivity and specificity of the PCR reaction. The optimized concentration of SYBR Green fluorescent dye enables fluorescence enhancement specifically after the SYBR Green fluorescent dye is incorporated in double-stranded DNA. Fluorescence can be measured during the annealing or extension phase.

This 2 x GeneScan Green Fast Mixture is a 2 x premix for fluorescence quantitative PCR reaction systems. The end user just needs to include template, primers, and water to make a 1 x concentration for the reaction to be carried out. This product is simple to use, has good stability, high sensitivity and specificity, and saves the end user time by minimizing human error, and reducing the chance of contamination.

Product ID Size
AG33261mL
AG3326-5ML 5mL
AG3326-15mL 50mL



Note: each product is suitable for the following models:

Thermal Cycler Dice Real Time System, LightCycler, Smart Cycler System, Corbett Rotor-gene6000, Mastercycler® eprealplex, LightCycler480, CFX96TM, CFX384TM fluorescence quantitative PCR instrument.

Precautions

1. To avoid foaming, mix gently by inverting up and down.

AG3326
$290.00 $264.99

2x GeneScan 2X SYBR Real-Time PCR Master Mix

N/A
In stock

Minimum quantity for "AmeriDx® 2X SYBR Green Real-Time PCR Master Mix, 1.0 mL/500RXNs" is 1.


ADX3069
$75.00
AmeriDx® PCR Ladder, 100 Loadings. 0.1 to 5 kb (100bp. 500bp, 1kb, 2kb, 5 kb).
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ADX3070
$55.00
Concentrated (10x) DNA or RNA loading dye for agarose gel electrophoresis. Two dyes as indicator: bromophenol blue and xylene cyanol FF.
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ADX0128
$20.00

5 M Betaine, 1.5 mL/Vial, PCR additive

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Minimum quantity for "AmeriDx® 5 M Betaine, 1.5 mL/Vial (Cat# ADX0128)" is 1.


ADX0126-100
$134.00 $74.00


High quality proteinase enzyme. It is designed for molecular diagnostics kits, including the COVID-19 nucleic acid extraction kit. Prevent viral nucleic acid from degradation. Both liquid and lyophilized powder format are provided to meet varied detection requirements of COVID-19/HCV/HBV.

In stock

Minimum quantity for "Recombinant Proteinase K, 100 mg lyophilized" is 1.


ADX0126-1ML
$70.00 $45.00

High quality proteinase enzyme. It is designed for molecular diagnostics kits, including the COVID-19 nucleic acid extraction kit. It can efficiently prevent viral nucleic acid degradation. Both liquid and lyophilized powder are provided to meet specific detection requirements of COVID-19/HCV/HBV.

In stock

Minimum quantity for "Recombinant Proteinase K, 1mL" is 1.


AG2058
$234.00 $145.00

AG2058, AmeriDx® Pre-stained Protein Marker II (10-200KD)

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AG2017-1L
$32.00 $27.29

AmeriDx® SDS-PAGE Transfer buffer, AG2017-1L

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AG0001-2L
$40.00 $34.06

Tris-Buffered Saline (TBS) Powder, final concentration for each component: 20 mM Tris, 150 mM NaCl, pH 7.6, AG0001-2L

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AG0002-2L
$40.00 $34.06

Phosphate-buffered saline (PBS) Powder, final concentration for each component: 137 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, AG0002-2L

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AG2086
$234.00 $145.00

AG2086, AmeriDx® Western blot protein marker I (Exposure), 250 uL/vial, 6 vials/pack

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AG2002
$65.00 $39.99

AG2002, AmeriDx® RIPA Lysis Reagent (strong)

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AG3020-250ML
$65.00 $39.99

Nucleic Acid remover (spray and clean working area/bench), AG3020-250ML

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AG3019-100ML
$80.00 $64.68

RNA solid tissue RNA stable preserving reagent (equivalent to RNA later), 100 mL/bottle, AG3019-100ML

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AG3029-10ML
$65.00

RNA elution/storage solution, , 1mL x 10, AG3029-10ML

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AG3606-100UL
$95.00

AmeriDx® Nucleic Acid Staining Reagent, AG3606-100UL

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AG3337
$300.00 $260.00

AmeriDx® First-Strand cDNA Synthesis Super Mix for qPCR(one-step gDNA remover), 50 rnx, AG3337

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AG3362
$65.00

AmeriDx® DNA Marker, 500 loadings, 500 uL/vial, AG3362

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