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AmeriDx® First-Strand cDNA Synthesis Super Mix for qPCR(one-step gDNA remover), 50 rnx, AG3337

AmeriDx® First-Strand cDNA Synthesis Super Mix for qPCR(one-step gDNA remover), 50 rnx, AG3337

List price: $300.00
$260.00
You save: $40.00 (13%)
AG3337
In stock
+

Description/Introduction

This product contains 5× Ready-to-Use Super Master Mix (reverse transcriptase cocktails including Reverse Transcriptase, RNase Inhibitor, Random Primer, Oligo (dT)18 Primer, dNTPs and the optimized buffers), No reverse transcriptase control (has the same compositions as Super Master Mix except the reverse transcriptase enzyme) and one step gDNA remover reagents. It can remove genomic remnants of the template before the reverse transcription reaction is performed. Reverse Transcriptase (RT) is a mutant reverse transcriptase obtained by in vitro evolutionary screening and is characterized by high thermal stability and processivity. gDNA Remover is a thermosensitive DNase that is 30 times more active than DNase I and works only on dsDNA, gDNA Remover is susceptible to inactivation under high temperature conditions.

Storage and Handling Conditions

Shipping with blue ice, stored at -20℃ upon received.

Component

Component Number

Component

G3333-50

G3333-100

AG3337

5× cDNA Super Mix for qPCR

200 μl

400 μL

AG3337-R

gDNA Remover

50 μL

100 μL

 

Nuclease-Free Water

1 mL

1 mL

G3337-C

5× No-RT Super Mix Control

20 μL

40 μL

Assay Protocol / Procedures

1. Synthesis of the first strand cDNA and Genomic DNA removal

(1) Reaction setup for 20 uL volume:

 

Component

Volume

5× cDNA Synthesis Super Mix for qPCR

4 μL

gDNA Remover

1 μL

Total RNA/mRNA

0.1 ng-5ug / 10 pg-0.5ug

Nuclease-Free Water

Add to 20 μL

(2) Gently mix and centrifuge.

(3) Setup reverse transcription program on a PCR machine

 

Temperature

Time

Primer annealing

25℃

5 min

cDNA synthesis

42℃

15-30 min

Enzyme Inactivation

85℃

5 s

Storage

10oC

varies



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