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RNA solid tissue RNA stable preserving reagent (equivalent to RNA later), 100 mL/bottle, AG3019-100ML

RNA solid tissue RNA stable preserving reagent (equivalent to RNA later), 100 mL/bottle, AG3019-100ML

List price: $80.00
$64.68
You save: $15.32 (19%)
AG3019-100ML
In stock
+

Description/Introduction

RNAsolidTM RNA Stabilization Solution is a tissue storage reagent that can quickly permeate the tissue to inhibit RNase activity and preserve RNA in fresh specimens. It is designed for preserving the collected samples that cannot be processed or frozen immediately. Simply submerge the samples in RNAsolidTM RNA Stabilization Solution and store at room temperature for later use.

This product has a wide range of applications, not only for fresh animal tissue such as liver, brain, lung, spleen, kidney, heart, muscle, etc., but also for RNA viruses, cultured cells, fungi, bacteria, blood and some plant tissues. It may not be effective in tissues that are poorly penetrated by the solution, such as waxy plant tissue and bone.

This product is safe and convenient, non-toxic and non-irritable, and can be used at room temperature. Most samples can be stored in RNAsolidTM RNA Stabilization Solution at 37°C for 1-2 days(25°C for 1 week, 4°C for 1 month, -20°C or lower temperature for 2-4 months)without significant RNA degradation.

Storage and Handling Conditions

RNAsolidTM RNA Stabilization Solution is shipped at room temperature; shelf life at room temperature is 24 months.

Components

Component Number

Component

G3019-100ML

G3019-1.2ML

G3019-1

RNAsolidTM RNA Stabilization Solution

100ML

70×1.2ML

Assay Protocol / Procedures

Sample handling

  • Use RNAsolidTM RNA Stabilization Solution for fresh tissue. Do not freeze tissues.
  • Cut the large tissue to a small piece (≤0.5 cm) for better preservation. Completely immerse the tissue in 5-10 volumes of RNAsolidTM RNA Stabilization Solution.
  • Most samples can be stored in RNA Stabilization Solution at 37°C for 1-2 days(25°C for 1 week, 4°C for 1 month, -20°C or lower temperature for a long time)without significant RNA degradation.
  • Tissue samples: Retrieve tissue from RNAsolidTM Solution with sterile forceps, quickly blot away excess RNAsolidTM Solution with paper towel, and then submerge the sample in RNA lysis solution. Proceed following the RNA extraction kit manual.
  • Cell and bacteria samples: pellet the samples, and proceed with RNA lysis buffer following RNA extraction kit manual.
    • The tissue samples should be quickly and completely immersed in RNAsolidTM RNA stabilization solution.
    • This product is suitable for the stabilization and preservation of RNA in fresh tissue or cells samples.
    • Tissues stored in this RNAsolidTM RNA Stabilization Solution are compatible with mainstream RNA extraction kits.
    • This product can also be used for stabilization and preservation of protein and DNA in tissues, however RNAsolidTM RNA Stabilization Solution will cause protein to denature.
    • After biopsy, samples treated with this RNA Stabilization Solution may become slightly hard. This won’t affect downstream procedures such as homogenization and RNA lysis.
    • For your safety and health, please wear lab coats and disposable gloves for sample processing.

Animal tissue: tissue that has been equilibrated in RNAsolidTM Solution can be removed from the solution, sectioned into smaller pieces, and put back to RNAsolidTM Solution. It may not be effective for tissues like animal bone, which has poor penatrability.

Plant tissue: Plant tissues that have natural barriers for solution diffusion (eg. waxy coatings on leaves), and will usually require physical disruption to allow RNAsolidTM Solution access to the tissue.

Whole blood sample: Collect 100-300 µL of fresh blood sample with the anticoagulant (EDTA sodium, potassium, heparin or citrate, etc.), mix with the solution at a ratio of 1:5.

Yeast, bacteria sample: Pellet the bacteria, and quickly mix with 2 volumes of this solution, and vortex.

Cell samples: Wash the cells with PBS, resuspend in 5-10 volumes of RNAsolidTM Solution.

Virus RNA samples: For swab collection, immerse the swab directly to 0.5-1 mL RNAsolidTM Solution. For liquids samples such as saliva, add 5-10 volumes of RNAsolidTM Solution to the collection tube, store at the appropriate temperature.

Sample preservation

For long term storage, immerse the sample in RNAsolidTM Solution at 4℃ overnight, then replace the RNAsolidTM Solution. Can be stored at -20℃ or -80℃ up to 5 years.



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