AmeriDx offers antibody cDNA cloning and over-expression of antibody in mammalian cell lines or bacterial host.
1, Start either with hybridoma cells or B cells from immunized animals, AmeriDx will clone the both heavy chain variable region (VH) and light chain variable region (VL) of monoclonal antibodies to the over-expression vector with your preference.
2, A small library will be constructed and screened.
3, High titer antibody expression colonies will be identified, and delivered to customers.
What does the customer provide to AmeriDx?
1. >= 105 cells per hybridoma cells, or spleen cells/tissue.
2. Antigen can be used for screening.
What does customer expect from AmeriDx?
1-5 stable cell line clones expressing high level of target antibody, and 100 ug recombinant antibody from each clone.(Catalog# ABC0316).