Warning Cookies are used on this site to provide the best user experience. If you continue, we assume that you agree to receive cookies from this site. OK

Protein Expression and Purification Services - no proteins, no charge

Protein Expression and Purification Services - no proteins, no charge

A0208181
In stock

Guaranteed 1 mg of purified soluble protein from $500 !

Introduction

ADX is pleased to launch the protein expression and purification service. ADX has know-how to deliver the best possible results to our valued customers. We have the capacity to take over your project from gene synthesis to protein expression and purification.

At ADX, we are proud of our capability to optimize protein expression, maximize product yield, and ensure the purity to serve every aspect of your research needs.

Protein Expression Systems at ADX

1. E. coli  Protein Expression System

Escherichia coli (E. coli) is widely used prokaryotic host for producing heterologous proteins.

The advantages about this system are:
        (1) low cost
        (2) fast turnaround time
        (3) well established purification procedures

Most of the proteins extracted from this prokaryotic system retain activity and function in vitro system confirmation.

2. Insect Cell Expression System 

This eukaryotic system is used for the producing precisely matured, folded and processed recombinant proteins. Compared with prokaryotic cells, insect cells are able to incorporate post-translational modifications, such as glycosylation. This unique system usually yields high amounts of the target proteins with correct confirmation, making it highly cost effective in comparison to mammalian cell expression systems, although the post-translation sometimes could be not 100% for human proteins.

3. Mammalian Expression System (Mammalian cell line, CHO, HEK 293, etc.)
The Mammalian Expression System is adopted for producing human or other mammalian origin proteins requiring post-translational modification for their in vitro functional assay (e.g. vaccines and enzymes,  as well as hormones and immunobiologically antibodies, interleukins, and lymphokines). Compared with bacterial host and insect cells, this system usually provides best biological activity of the recombinant proteins.

Services and Price Summary

Service Name

Details

Deliverables

Price

E coli.

Guaranteed 1mg purified soluble protein
(Cat# APXPEC-1MG)

  • Subcloning
  • Expression optimization and verification
  • Expression and purification
  • QC by SDS-PAGE, Western blot*
  • Tag removal, endotoxin removal service available

Guaranteed 1 mg purified soluble protein

From $500
Request Service, 1mg

E coli.

Guaranteed 5 mg purified soluble protein
(Cat# APXPEC-5MG)

  • Subcloning
  • Expression optimization and verification
  • Expression and purification
  • QC by SDS-PAGE, Western blot*
  • Tag removal, endotoxin removal service available

Guaranteed 5 mg purified soluble protein

From $2050
Request Service, 5mg

Insect cells

(Cat# APXP-S2)

  • Subcloning
  • Generation of recombinant vector DNA
  • Establish stable insect cell line or baculovirus
  • Expression and purification of target protein
  • QC by SDS-PAGE, Western blot*
  • Tag removal service available

Purified soluble protein

Please Inquire
Request Service

Mammalian cells

(Cat# APXP-MAMM)

  • Transient expression and purification
  • Or stable cell line establishment
  • Expression and purification of target protein
  • QC by SDS-PAGE, Western blot*
  • Tag removal service available

Purified protein

Please inquire
Request Service

* Western blot, the customer provides antibody to target protein or ordering information, and ADX will purchase the antibody for the customer.

These Packages have the following features:

  • Customer provides the plasmid or the expression strain/cell lines harboring target gene.
  • Size of the target protein is usually under 100 kDa.
  • In some rare cases, in order to facilitate expression, ADX may try different tags and the final protein product may contain such tag
    The final purified protein is tag-containing.
  • ADX will remove most of the toxin from the protein extraction by multiple chromatographic columns, however ADX does not guarantee the endotoxin level of purified protein.
  • ADX guarantees that purified proteins to be soluble, as for the bio-reactivity and functionality, it is depended on individual proteins. If target protein expresses as inclusion body, ADX will refold it and make it soluble before delivery.

Please filled in this form if you have any protein expression and purification project for us.


21232499

Western blot services, from protein sample or sequence to publishable images.

In stock

ADX00001
ADX offers standard DNA cloning, subcloning and site specific mutagenesis services, please inquiry . Target Gene Size Deliverable...
In stock

ES072716A
ELISA Testing Services
In stock

A0208181B

Protein Expression and Purification Services - Bacterial Host

In stock

A0208181IC

Protein Expression and Purification Services - insect cells

In stock

A0208181M

Protein Expression and Purification Services, mammalian cells

In stock