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Protein Expression and Purification Services -- mammalian cells, no protein, no charge

Protein Expression and Purification Services, mammalian cells

A0208181M
In stock

Mammalian Expression System (Mammalian cell line, CHO, HEK 293, etc.)

The Mammalian Expression System is adopted for producing human or other mammalian origin proteins requiring post-translational modification for their in vitro functional assay (e.g. vaccines and enzymes,  as well as hormones and immunobiologically antibodies, interleukins, and lymphokines). Compared with bacterial host and insect cells, this system usually provides best biological activity of the recombinant proteins.

Services and Price Summary

Mammalian cells

(Cat# APXP-MAMM)

  • Transient expression and purification
  • Or stable cell line establishment
  • Expression and purification of target protein
  • QC by SDS-PAGE, Western blot*
  • Tag removal service available

Purified protein

Please inquire
Request Service

* Western blot, the customer provides antibody to target protein or ordering information, and ADX will purchase the antibody for the customer.

These Packages have the following features:

  • Customer provides the plasmid or the expression strain/cell lines harboring target gene.
  • Size of the target protein is usually under 100 kDa.
  • In some rare cases, in order to facilitate expression, ADX may try different tags and the final protein product may contain such tag
    The final purified protein is tag-containing.
  • ADX will remove most of the toxin from the protein extraction by multiple chromatographic columns, however ADX does not guarantee the endotoxin level of purified protein.
  • ADX guarantees that purified proteins to be soluble, as for the bio-reactivity and functionality, it is depended on individual proteins. If target protein expresses as inclusion body, ADX will refold it and make it soluble before delivery.

Please filled in this form if you have any protein expression and purification project for us.


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