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Protein Expression and Purification Services - from Bacterial Host, no protein no charge

Protein Expression and Purification Services - Bacterial Host

A0208181B
In stock

AmeriDx Protein Purification Services -- Bacterial hosts

Introduction

Bacteria are the most common hosts for recombinant protein over-expression and purification, provided that construction of expression vector for bacteria is fast and easy and transfer to bacteria, especially gram negative bacteria is a basic molecular means in regular biology laboratories. Most common bacteria host is E coli, for example BL21 strain is widely used in heterogenic proteins over-expression and purification, due to its low proteinase activity facilitating the foreign protein accumulation inside host cells. However, other gram negative strains like P aeruginosa and P. putitda, and gram positive strain S. subtilities also are adopted in some occasions. 

AmeriDx has two common bacterial hosts for heterogenic protein expressions: E coli BL21 strain and P. putida stain, which increase the success rate for recombinant protein purification.

Bacterial Protein Expression Systems at ADX

1. Two Promoters for Bacterial Protein Expression Systems

The vectors for BL21 strain include IPTG inducible T7 promoter and APX inducible promoter T5. IPTG switch is simply on and off, once it is on, the protein is translated and accumulated inside the cell plasma, and easy to form inclusion body to eliminate its impact to host cells. On the contrary, APX 

2. Screening Right Colonies

AmeriDx has large set of screening library for screening high soluble and active target protein clones, and maximize the protein yield and make sure the protein in active form.

3. Two-Step Purification Procedure - Guarantee the purity

after cell lysis, the protein extraction runs again an affinity column (myc, his tag or GST), then by a size exclusion column to remove any non target proteins.

Services and Price Summary

Service Name

Details

Deliverables

Price

E coli.

Guaranteed 1mg purified soluble protein
(Cat# APXPEC-1MG)

  • Subcloning
  • Expression optimization and verification
  • Expression and purification
  • QC by SDS-PAGE, Western blot*
  • Tag removal, endotoxin removal service available

Guaranteed 1 mg purified soluble protein

From $500
Request Service, 1mg

E coli.

Guaranteed 5 mg purified soluble protein
(Cat# APXPEC-5MG)

  • Subcloning
  • Expression optimization and verification
  • Expression and purification
  • QC by SDS-PAGE, Western blot*
  • Tag removal, endotoxin removal service available

Guaranteed 5 mg purified soluble protein

From $2050
Request Service, 5mg

* Western blot, the customer provides antibody to target protein or ordering information, and ADX will purchase the antibody for the customer.

These Packages have the following features:

  • Customer provides the plasmid or the expression strain/cell lines harboring target gene.
  • Size of the target protein is usually under 100 kDa.
  • In some rare cases, in order to facilitate expression, ADX may try different tags and the final protein product may contain such tag
    The final purified protein is tag-containing.
  • ADX will remove most of the toxin from the protein extraction by multiple chromatographic columns, however ADX does not guarantee the endotoxin level of purified protein.
  • ADX guarantees that purified proteins to be soluble, as for the bio-reactivity and functionality, it is depended on individual proteins. If target protein expresses as inclusion body, ADX will refold it and make it soluble before delivery.

Please filled in this form if you have any protein expression and purification projects for us.


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